| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Leptospira biflexa | GGCTTCGATGTCAGCAGGT | AGGGTTTGCGAATGGCCA | 60.08 | 59.88 | 264 | 117 |
100.00%
|
100.00%
|
| Primer 222 | Leptospira biflexa | CGGGACACGCGTATTGGAT | GCGCACTGCTTTGCTGAA | 60.23 | 59.67 | 269 | 117 |
100.00%
|
100.00%
|
| Primer 223 | Leptospira biflexa | CTCACACGCATTGGGACCA | GCGCACTGCTTTGCTGAA | 60.30 | 59.67 | 245 | 117 |
100.00%
|
100.00%
|
| Primer 224 | Leptospira biflexa | ACTCACACGCATTGGGACC | GCGCACTGCTTTGCTGAA | 60.30 | 59.67 | 246 | 117 |
100.00%
|
100.00%
|
| Primer 225 | Leptospira biflexa | TCACACGCATTGGGACCAT | GCGCACTGCTTTGCTGAA | 59.62 | 59.67 | 244 | 117 |
100.00%
|
100.00%
|
| Primer 226 | Leptospira biflexa | GGATCCTCACTCACACGCA | GCGCACTGCTTTGCTGAA | 59.41 | 59.67 | 254 | 117 |
100.00%
|
100.00%
|
| Primer 227 | Leptospira biflexa | AGGAAGGAGGGACAGAAGCT | CCGGTTTTCCATGGCATTGG | 59.88 | 60.11 | 288 | 117 |
100.00%
|
100.00%
|
| Primer 228 | Leptospira biflexa | GCGACTGGGACAACAGGAA | GGTCATGGGGTTGTGCTGA | 59.93 | 59.93 | 169 | 117 |
100.00%
|
100.00%
|
| Primer 229 | Leptospira biflexa | GCGTTTGGTGCTTGGTGAG | ACGCGTCTTTTGTGGGGT | 60.01 | 59.81 | 236 | 117 |
100.00%
|
100.00%
|
| Primer 230 | Leptospira biflexa | GCGTTTGGTGCTTGGTGA | ACGCGTCTTTTGTGGGGT | 58.88 | 59.81 | 236 | 117 |
100.00%
|
100.00%
|